Reducing Cellular Autofluorescence
نویسندگان
چکیده
منابع مشابه
Reducing cellular autofluorescence in flow cytometry: an in situ method.
Cellular autofluorescence affects the sensitivity of flow cytometric assays by interfering with detection of low level specific fluorescence. These detection limits increase with use of protocols, such as thermocycling and fluorescent in-situ hybridization (FISH), that can increase intrinsic cellular fluorescence to 5,000-20,000 fluorescein isothiocyanate (FITC) equivalents. In order to improve...
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Introduction The ability to track fluorescent proteins, dyes, quantum dots, and other fluorescent reporters in vivo is a rapidly growing technology known as molecular imaging. The IVIS Imaging Systems developed at Xenogen are optimized for imaging both bioluminescent and fluorescent reporters in vivo. Fluorescence equipment is standard on the IVIS Imaging System 200 Series while the IVIS Imagin...
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A. Holloschi, HM. Kuhn, C. Müller, M. Worf, M. Rauen, T. Röder, W. Kessler, J. Mollenhauer and P. Kioschis 1 University of Applied Sciences Mannheim, Institute of Molecular and Cell Biology and Institute of Chemical Process Engineering, Mannheim, Germany 2 Steinbeis-Hochschule Berlin, Steinbeis-Insitut Multivariate Datenanalyse, Berlin, Germany 3 University of Southern Denmark, Lundbeckfonden C...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1998
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500069765